Sometimes disabling & re-enabling Explorer replacement can solve things if registry settings have been modified by something else.
Reinstalling the current version of Opus over the top of itself is also worth a try, in case the issue is a DLL that has become unregistered or similar.
Anti-virus or firewall tools may be blocking Chrome from talking to Opus (and web browsers from talking to anything other than File Explorer in general, perhaps). Disabling them is worth a try.
The TestExplorerReplacement.zip archive in Re: Visual Studio + Open Folder in File Explorer can be used to check if the API Chrome uses is working in general, which will tell you if the problem is specific to Chrome (which would make an antivirus/firewall tool the most likely cause) or general to all software (which leaves things more open-ended).