Dopus taskbar Icon not appearing

Did you check the preferences items Tanis showed anyway?

If they are set as in the screenshot then everything should be normal.

Does it only happen when a particular program is in use, or when a particular program is above Opus?

Are you using an alternative desktop/taskbar replacement?

Are your Opus windows pinned?

A screenshot showing your full desktop including the other app and the taskbar might help shed some light on what is happening.