So, so far, all we know is there is sometimes an error when copying large amounts of data between two of your harddrives. There's nothing to suggest it isn't a legitimate error coming from the operating system, caused by a problem that that would affect the same copy if it was done using Explorer instead of Opus.
New drives fail all the time so a drive being new doesn't mean there's no chance of it having bad sectors or problems reading/writing when hot, and so on. Leaving a full chkdsk surface scan to run over night seems like a good idea to me. That will make sure the disks are okay and your data is safe, and means we can rule that possibility out if no problem is found.
Another fairly common cause of data corruption when reading/writing a lot of data, and particularly when also drawing to the screen (or playing sound or, basically, accessing other things on the PCI/AGP bus) like Opus's progress dialog does, is IDE/motherboard driver and firmware bugs. This has affected just about every manufacturer over the years and still crops up in recent hardware. It usually gets fixed once the manufacturers notice, but if your IDE/motherboard drivers/firmware are not up-to-date then it's definitely worth installing the updates. I've personally seen problems like this with VIA, NVidia and Intel chipsets, all of which got fixed by later firmware and/or driver updates. That includes my current PC whose model of motherboard, that was only released about a year ago, initially had data corruption issues until an updated firmware came out
It's up to you, of course. It's your data and time. So far though, and not speaking on behalf of GPSoftware (who I don't work for or represent), I don't see anything to investigate in Opus. I've copied hundreds of gigs of data (at a time) between various types of HDD on many occasions using Opus without any problems. It seems like you're seeing error messages and assuming the problem is the program reporting the errors, with no investigating into the cause of them or whether they are due to a real problem. Maybe there is a bug in Opus that causes error messages about corrupt files/folders if you copy a lot of data, but this is the first time anyone has reported it, we don't have much to go on to investigate or try to reproduce it it, and there seem to be a lot of other things to look into on your computer that are more likely to be the cause of the problem. If there is a data corruption problem is best found sooner than later.