At the moment toolbars can't be saved as part of layouts or anything else.
There is a set of toolbars which are on for every single lister and in the same place for every single lister. These are the toolbars which are ticked in the Customize dialog.
You can also open "local" toolbars which are only in a single lister by using the Toolbar command with the LOCAL argument.
You can create a button which opens a new lister (or layout) and then turns on a local toolbar in it. There isn't a perfect way to do this at the moment but there is a way which seems to work reasonably well: