Drive partition help

The original computer setup was C:\ win 10 boot & D:\ Audio
After cloning and installing a new SSD, there are now 3 drive letters instead of two. Audio drive got assigned to F:
Partition wizard reads the drives correctly, but DOPUS
Also i'm not sure why the boot disk is not Drive 1.

Opus should show the same thing as File Explorer and every other non-partitioning tool.

Opus doesn't determine the drive letters or order; Windows does. But partitioning tools may view things in a different way, since they work at a different level.

Assuming you see the same thing in File Explorer, everything is normal.

fixed it by using miniTools Partition wizard free, "remove" drive letter" and reassigning D to the audio HDD.

You should be able to do that kind of thing via Computer Management / Disk Management in Windows, FWIW. What that shows should be authoritative, since it's part of the OS.