Mystery drive letter in folder tree (Opus 10)

Strange that my method with MiniTool do not work in your case. Are you sure that you click "Apply" after every operation? MiniTool first just shows you what you want to do and after "Apply" execute your jobs. You must click "Apply" after each job (assign letter, remove letter etc.).

Or - you can remove all entries from "MountedDevices" and Windows write correct ones after reboot.