Non-Existent Folders from Last Computer Still Showing

I'm running V12 of DO under Windows 10. I've recently moved to a new computer and saved and restored my DO settings from my old computer. Whenever I open up DO in Dual Horizontal mode it defaults to two listersshowing folder paths that no longer exist on my new computer. How do I get rid of this?

Adapt the lister to your liking and save its Layout and/or set it as the default lister.