Is it possible to set twin panes to be identical in format?

For getting the same columns and widths in all folders (and on both sides): Folder Formats: Quick Guide

To make both sides equal size, double-click the splitter between them to auto-size it. The size would normally then be saved when you close the window. (Unless that is turned off, in which case use Settings > Set as Default Lister to save it explicitly. That also saves the current folders/tabs as the defaults for new windows.)